发明名称 Process for the redox potential dependent detection of target molecules through interacting polypeptides
摘要 <p>Nucleic acid molecule (I) is new, where (I) is a sequence (II) coding for a defined 94 residue amino acid sequence given in the specification, is complementary to (II), hybridizes to (II), is sufficiently homologous to (II), its complement or sequences hybridizing to (II) to be a functional analogue, is degenerate to, or is modified by deletion, addition, substitution, translocation, inversion or insertion from, any of the previous sequences. Independent claims are also included for the following: (1) polypeptide (III) encoded by (I); (2) polypeptide library containing (III); (3) a method (M1) for detecting and producing polypeptides binding to target molecules comprising immobilizing a potential target molecule on a carrier, bringing (III) in contact with the immobilized target molecule, elimination of the unbound (III) and elution of the bound (III) under stringent conditions; (4) a method for detecting target molecules whose inhibition is adjustable dependent on redox potential, comprising preparing a sample containing at least one candidate target molecule, bringing (III) in contact with the sample and detection of the interaction, particularly binding, between the target molecule and (III); and (5) a kit containing (I), (III) or a polypeptide library containing (III).</p>
申请公布号 EP1695981(A1) 申请公布日期 2006.08.30
申请号 EP20050090045 申请日期 2005.02.25
申请人 FORSCHUNGSVERBUND BERLIN E.V. 发明人 FREUND, CHRISTIAN;ZIMMERMANN, JUERGEN
分类号 C07K14/435;G01N33/50 主分类号 C07K14/435
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