| 摘要 |
The object of the present invention is to provide a polypeptide which can be used to produce a saccharide having a structure of cyclo{->6)-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->}, a DNA encoding the polypeptide, and uses thereof. The present invention solves the above object by establishing a polypeptide which has an enzymatic activity to produce a saccharide having a structure of cyclo{->6}-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->} from a saccharide with a glucose polymerization degree of 3 or higher and bearing both the alpha-1,6 glucosidic linkage as a linkage at the non-reducing end and the alpha-1,4 glucosidic linkage other than the linkage at the non-reducing end by catalyzing the alpha-isomaltosyl-transfer, and having an amino acid sequence of either SEQ ID NO:1 or SEQ ID NO:2, or that which is a member selected from the group consisting of amino acid sequences having deletion, replacement, or addition of one or more amino acid residues therein or thereto, a DNA encoding the polypeptide, and uses thereof.
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