CELL LINES FOR PRODUCTION OF REPLICATION-DEFECTIVE ADENOVIRUS
摘要
The present invention provides cell lines for the production of E1-deleted adenovirus (rAd) vectors that complement E1A and E1B functions. The present invention also provides cell lines for the production of E1- and E2-deleted adenovirus vectors that complement E1A, E1B and E2B polymerase functions. The invention provides particular cell lines that complement E1A function by insertion of an E1A sequence containing mutations in the 243R and 289R proteins and an E1B sequence comprising the E1B-55K gene. Production yields in the resulting producer cell lines, designated SL0003 and SL0006, were similar to those obtained from 293 cells without generation of detectable recombinant replication competent adenovirus ("RCA").
申请公布号
WO2006065827(A9)
申请公布日期
2006.08.24
申请号
WO2005US45097
申请日期
2005.12.12
申请人
CANJI, INC.;HOWE, JOHN, A.;SHERRILL, SCOTT, J.;WILLS, KEN;RALSTON, II, ROBERT, ORVILLE
发明人
HOWE, JOHN, A.;SHERRILL, SCOTT, J.;WILLS, KEN;RALSTON, II, ROBERT, ORVILLE