发明名称 |
BIOSENSORS FOR MONITORING RECEPTOR-MEDIATED G-PROTEIN ACTIVATION |
摘要 |
<p>The present invention relates to novel biosensors that are based on bioluminescence resonance energy transfer (BRET). These biosensors may be used to monitor rapid interaction and conformational changes within G protein-coupled receptor/G protein complexes and, in this way, reflect the activation status of the receptor. Advantageously, the biosensors may be used as a highly sensitive and quantitative assay for the identification of ligands (agonists, antagonists, inverse agonists, partial agonists, etc.) targeting G protein-coupled receptors (GPCRs) as well as for the analysis of the activation status of these receptors. Moreover, multiplexing different biosensors within receptors/G protein complexes allows for mapping ligand textures. Additionally, the biosensors permit the direct, real-time examination of interactions between receptors and G protein in their natural environment, the living cell.</p> |
申请公布号 |
WO2006086883(A1) |
申请公布日期 |
2006.08.24 |
申请号 |
WO2006CA00233 |
申请日期 |
2006.02.16 |
申请人 |
UNIVERSITE DE MONTREAL;BOUVIER, MICHEL;GALES, CELINE;BRETON, BILLY |
发明人 |
BOUVIER, MICHEL;GALES, CELINE;BRETON, BILLY |
分类号 |
C12Q1/66;G01N21/76;G01N33/50;G01N33/542;G01N33/58 |
主分类号 |
C12Q1/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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