| 摘要 |
The invention relates to a method for the reproducible measurement and quantification of intracellular objects, for example organelles such as nuclei, mitochondria, etc., or other small objects such as lipid droplets or vesicles, which involves the following stages: staining the cells with an appropriate dye, photographing the cells with enlargement, digitalisation of the photographic images and analysis of the said image with a computer program. In accordance with the invention, the analysis stage comprises the following steps: analysis of the colours on the pixels of the digital image and the arrangement of pixels whose wavelengths have values which lie between certain predetermined upper and lower limit values, with adjacent pixels whose wavelengths also have values which lie between these predetermined upper and lower limit values, forming specific objects from the aforementioned combined pixels, and registering the number of the aforementioned specific objects. The invention also relates to an arrangement.
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