Verfahren und Vorrichtung für die Abgabe von Flüssigkeiten

摘要

A multiwell plate (30) for microarraying comprising a self-sealing lower membrane (14) and optionally also a self-sealing upper membrane (20) containing a liquid (18) to be dispensed. Spotting is performed by pushing a pin (10, 10') down through the liquid and then on to pierce the self-sealing lower membrane. A slide (16) having an upper spotting surface is arranged under the well plate. The liquid sample forced through the lower membrane by the pin tip can thus be deposited directly onto the spotting surface. The pin is then withdrawn upwards through the lower membrane, which automatically reseals preventing further loss of liquid. The optional resealable upper membrane also prevents loss of sample liquid by evaporation and spillage. By contrast to the prior art, there is the major advantage that the pin head does not have to traverse between the well plate and slide to collect sample liquid, thus dramatically increasing operational speed for an automated microarraying apparatus employing the multiwell plate. Excellent spot reproducibility is also observed.