| 摘要 |
A method for recognizing dark states during the spectroscopic or microscopic examination of fluorescent specimens ( 1 ), in particular using fluorescent proteins, the excitation/illumination volume, thus the intensity distribution of the excitation light, being varied over the course of at least two mutually independent measurements, a determination being made as to whether the observed time constants change and, in the case of unchanged time constants, the existence of a dark state being inferred.
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