| 摘要 |
The present invention provides an RNA amplification process, comprising steps in which double-stranded DNA containing a promoter sequence is produced using a first primer and second primer, at least one of which has the promoter sequence at the 5' end, and reverse transcriptase, the double-stranded DNA is used as template for production of RNA transcript using RNA polymerase and the RNA transcript is used as template for subsequent DNA synthesis with the reverse transcriptase to produce the double-stranded DNA. The process yields an amplified RNA product which is assayed with a fluorescent intercalator dye-labeled nucleic acid probe. |
