| 摘要 |
The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X<SUB>1</SUB>-X<SUB>2</SUB>-X<SUB>3</SUB>-X<SUB>4</SUB>-X<SUB>5</SUB>- where X<SUB>1 </SUB>is phenylalanine, tryptophan, or tyrosine, X<SUB>2 </SUB>is isoleucine, leucine, valine, alanine or methionine, X<SUB>3 </SUB>is leucine, valine, alanine or methionine, X<SUB>4 </SUB>is serine or threonine and X<SUB>5 </SUB>is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.
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