COFOCAL MICROSCOPE

摘要

A Nipkow system cofocal microscope in which a region of a sample can be illuminated selectively and observed using a DMD. Illumination light from a light source (20) passes through a condenser lens (1) and a rotary filter (14) and impinges on a DMD (2) and only the light subjected to on-control by the DMD (2) and illuminating a micro mirror region impinges on the microlens (5) of a Nipkow disk assembly. The light passed through the microlens (5) is converged by a pinhole (6) to form an image in a selected region of a sample (10) by an objective lens (8). Reflected light, e.g. fluorescence, from the selected region of a sample (10) retrogresses along the incident optical path and is reflected by a dichroic mirror (7) before an image is formed on a two-dimensional photodetector by an image forming lens (11).