| 发明名称 |
Derivatized versions of ligase enzymes for constructing DNA sequences |
| 摘要 |
A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.
|
| 申请公布号 |
US7090979(B2) |
申请公布日期 |
2006.08.15 |
| 申请号 |
US20030718856 |
申请日期 |
2003.11.21 |
| 申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
MARIELLA, JR. RAYMOND P.;CHRISTIAN ALLEN T.;TUCKER JAMES D.;DZENITIS JOHN M.;PAPAVASILIOU ALEXANDROS P. |
| 分类号 |
C12Q1/68;C12N15/10;C12P19/34 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
|
| 地址 |
|
