A PURIFICATION AND CULTIVATION METHOD OF OCEAN SPONGE PLURIPOTENT STEM CELL

摘要

A purification and cultivation method of ocean sponge pluripotent stem cell, which can based on the size and adhesion nature of the spongy cell to separate and purify the said sponge cell by differential centrifugation, differential aggregation, differential adhesion and density gradient centrifugation in turn, the said sponge cell has been pre-treated. To add the growth factor and differentiation control factor at the cultivation step of the present invention, can facilitate the proliferation of the pluripotent stem cell and inhibit its differentiation. After being separated, the activity of the obtained pluripotent stem cell is above 95%; The isolated and purified sponge pluripotent stem cell of the present invention can be used in the large scale cultivation to achieve the biosynthesis of active material.