СПОСОБ ЗАЩИТЫ ОТ-ПЦР ОТ КОНТАМИНАЦИИ ПРОДУКТАМИ АМПЛИФИКАЦИИ НА ОСНОВЕ РАЗРУШАЮЩЕГО ДЕЙСТВИЯ УРАПЦИЛ ДНК ГЛИКОЗИЛАЗЫ

摘要

FIELD: molecular biology, in particular detection of biological objects. ^ SUBSTANCE: claimed method includes addition of uracyl-DNA-glycosylase in mixture for reverse transcriptase reaction and carrying out of reverse transcriptase and polymerase chain reaction in single reaction volume. ^ EFFECT: high effective protection of RT-PCR from contamination by amplification products. ^ 1 tbl, 2 dwg