METHODS FOR DETERMINING RESPONSIVENESS TO CANCER THERAPY

摘要

In certain aspects, the present invention provides methods and compositions for determining whether a cancer cells is likely to respond to treatment with a Her 1 -acting agent. In one aspect, the invention provides a method for determining whether a cancer cell is likely to respond to treatment with a Her 1 -acting agent, comprising detecting on the cancer cell at least about 600 Her 1 -Her 1 dimers, wherein the presence of the at least about 600 Her 1 -Her 1 dimers indicates that the cancer is likely to respond to treatment with the Her 1 -acting agent. In another aspect, the invention provides a method for determining whether a cancer cell is likely to respond to treatment with a Her 1 -acting agent, comprising detecting on a cell of the cancer at least about 600 Her 1 -Her 1 dimers, at least about 1000 Herl-Her2 dimers, and fewer than about 1000 Her2-Her3 dimers, wherein the presence of the at least about 600 Her 1 -Her 1 dimers, the at least about 1000 Herl-Her2 dimers, and the fewer than about 1000 Her2-Her3 dimers indicates that the cancer cell is likely to respond to treatment with the Her 1 -acting agent. Preferably, methods of the invention are implemented by using sets of binding compounds having releasable molecular tags that are specific for multiple components of one or more types of receptor dimers. After binding, molecular tags are released and separated from the assay mixture for analysis.