METHOD FOR DETERMINING THERMAL STABILITY OF COLLAGEN OR COLLAGEN-LIKE PEPTIDE

摘要

The present invention is a method for determining the thermal stability of a collagen peptide, collagen-like peptide or triple-helix construct with the repeating peptide unit Gly-Xaa<SUB>1</SUB>-Xaa<SUB>2</SUB>. The instant method accounts for the destabilizing effect of peptide repeats which do not conform to the highly stable Gly-Pro-Hyp peptide and for the interaction between triplets. The instant method finds use in mutant analysis of collagen peptides, collagen-like peptides or triple-helix constructs and engineering of collagen peptides, collagen-like peptides or triple-helix constructs.