POLYCLONAL ANTIBODY SPECIFICALLY REACTING WITH PRIONIC PROTEIN IN CEREBRAL TISSUE OF MAMMALIANS AND DIAGNOSTIC REAGENT UPON ITS BASIS

摘要

FIELD: biotechnology. ^ SUBSTANCE: the suggested innovation deals with a polyclonal antibody to new synthetic fragment 143-168 SEQ ID NO:1 of bovine prionic protein followed by obtaining high-reactive rabbit's antisera without conjugation of peptides with a carrier, their purification upon an affine sorbent to obtain highly purified antibodies and creating diagnostic reagent for detecting prionic protein due to "sandwich"-technique of IEA in cerebral tissue of mammalians by applying a couple "antibody-conjugate" including antibody to peptide 143-168 and antibody conjugate to peptide 211-241 with horseradish peroxidase. ^ EFFECT: higher efficiency of detection. ^ 7 cl, 8 ex, 6 tbl