| 摘要 |
A purification process for the capsular polysaccharide of S.agalactiae in which the saccharide is initially treated with an aqueous mixture of an alcohol and a calcium salt, followed by precipitation with a cationic detergent. The process can be completed in less than three days and has a yield of around 60%. It avoids the need for DNase, RNase and/or protease treatment. The saccharides of the process have a very low protein contamination and a very low absorbance at 280nm. |
