Polyamide-oligonucleotide derivatives, their production and use

摘要

Polyamide-oligonucleotide derivatives (I) and their physiologically acceptable salts. Polyamide-oligonucleotide derivatives of formula F((DNA)-Li)q(PNA-Li)r(DNA-Li)s(PNA)t)xF' (I) and their physiologically acceptable salts are new. q, r, s, t = 0 or 1, with the sum of two or more adjacent letters at least 2; x = 1-20; DNA = nucleic acid (such as DNA or RNA or their known derivatives); Li = covalent linkage between DNA and PNA, i.e. a bond or a residue containing at least one atom of carbon, nitrogen, oxygen or sulfur; PNA = polyamide structure containing at least one nucleobase different from thymine; and F, F' = end groups and/or are connected through a covalent bond. Independent claims are also included for the following: (1) method for preparing (I); (2) pharmaceutical composition containing (I); and (3) gene probe assays for detecting oligo- or poly-nucleotide targets (RNA or DNA) in which (I) is used as probe and primer.