摘要 |
PROBLEM TO BE SOLVED: To provide a method for accurately quantifying the nuclear migration level of a nucleic acid transferred into a cell. SOLUTION: The method comprises (a) the step of fractionating a nuclear fraction from a cell transferred with a cationic conjugate comprising the objective nucleic acid and a cationic substance, (b) the step of washing the nuclear fraction fractionated in the step(a) to remove a cationic conjugate adhered to nuclear membrane and (c) the step of quantifying the objective nucleic acid contained in the nuclear fraction washed in the step(b). COPYRIGHT: (C)2006,JPO&NCIPI
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