发明名称 |
METHODS AND COMPOSITIONS FOR IMPROVING FIDELITY IN A NUCLEIC ACID SYNTHESIS REACTION |
摘要 |
The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3' hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide. |
申请公布号 |
WO2006081583(A2) |
申请公布日期 |
2006.08.03 |
申请号 |
WO2006US04638 |
申请日期 |
2006.01.30 |
申请人 |
HELICOS BIOSCIENCES CORPORATION;BUZBY, PHILIP, RICHARD |
发明人 |
BUZBY, PHILIP, RICHARD |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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