发明名称 |
CO-EXPRESSION OF MULTIPLE PROTEIN CHAINS OR SUBUNITS |
摘要 |
A recombinant genetic construct is provided that includes at least two expression cassettes. Each cassette encodes for a chain or subunit of a target protein. The genetic construct preferably targets any expressed protein to the secretory pathway of the host cell. An application of present invention is found in expressing the two chains of human insulin through two separate expression cassettes on the same methylotrophic yeast expression vector. Mature, bioactive human insulin molecules are secreted through this method without resorting to any post-translational cleavage process.
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申请公布号 |
WO2006052363(A3) |
申请公布日期 |
2006.08.03 |
申请号 |
WO2005US36479 |
申请日期 |
2005.10.11 |
申请人 |
GENOTEINS PHARMACEUTICAL INC.;MA, YANGAO;MA, XIANGDONG |
发明人 |
MA, YANGAO;MA, XIANGDONG |
分类号 |
C12N15/85;A61K38/16;A61K38/19;A61K38/28;A61K47/06;C07H21/04;C07K14/62;C12N5/10;C12N15/17;C12N15/63;C12N15/64;C12N15/81;C12P21/00;C12P21/02;C12P21/04 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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