摘要 |
It is an object of the present invention to develop an inexpensive and simple method for transcription and synthesis of siRNA. The present invention provides an oligonucleotide, which at least comprises, in a direction from the 5'-terminus to the 3'-terminus: (1) an antisense sequence of a target nucleic acid sequence; (2) a trimming sequence which is cleaved with base-specific RNase; (3) a sense sequence of a target nucleic acid sequence; (4) an antisense sequence of a promoter sequence; (5) a sequence that forms a loop; and (6) a sense sequence of a promoter sequence, wherein the above-described antisense sequence and sense sequence of a promoter sequence form a double strand in a molecule via a hairpin structure, and when DNA is transcribed, a transcriptional product from the above-described antisense sequence and sense sequence of a target nucleic acid sequence forms a double strand in a molecule via the trimming sequence.
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