| 摘要 |
a 1-antitrypsin is isolated from an a 1-antitrypsin-containing fraction obtained by ammonium sulphate precipitation from serum or ascitic liquid as described in Z. Naturforschung 10b, 463, 1955 by the following process (a) a compound of the acridine or quinoline series is added to the said fraction at a pH of 5.5 to 6.5 in a practically salt-free medium and at a concentration of said fraction in the range 1 to 6 per cent, (b) the addition is continued until the proteins which accompany a 1-antitrypsin are precipitated, (c) the precipitate is rejected, (d) the a 1-antitrypsin is precipitated from the liquid portion by increasing the pH value up to 6.5-7.5 and also the concentration of the said compound of the acridine or quinoline series, (e) the precipitate obtained is isolated and dissolved in water, (f) the acridine or quinoline base is precipitated from the solution prepared in (e) at a pH 4.0 to 5.5 by means of an inorganic salt, (g) the pH value of the a 1-antitrypsin solution is adjusted to pH 5.0 to 6.0, (h) the a 1-antitrypsin is precipitated at -1 DEG C. to -5 DEG C. by the addition of a water-miscible aliphatic alcohol up to a content in the medium of 40-50 per cent, (i) the precipitated a 1-antitrypsin is separated and dissolved in water and the organic precipitant adhering to the precipitate is removed by dialysis and the a 1-antitrypsin solution so formed is concentrated and/or lyophilized. The compound of step (a) may be 2-ethoxy-6, 9-diamino acridine lactate, bis-(2-methyl-4-amino-quinolyl-6)-carbamide hydrochloride, 3, 6-diamino-10-methyl acridinium chloride, 5-aminoacridine, a ,g -trimethylene glycol di-(2-methyl-4-aminoquinolyl)-6 ether diacetate or di-n-butylmalonic acid amide-N-N-di-(2-methyl-4-aminoquinolyl-6)-diacetate. a 1-antitrypsin is a therapeutic glycoprotein which occurs in the blood and is an inhibitor for proteolytic ferments.
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