LI-KEY/ANTIGENIC EPITOPE HYBRID PEPTIDE VACCINES

摘要

Disclosed is an antigen presentation enhancing hybrid polypeptide which includes three elements. The first element is an N-terminal element consisti ng essentially of 4-16 residues of the mammalian li-Key peptide LRMKLPKPPKPVSKM R (SEQ ID NO: _) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity. The second element is a chemical structure covalently linking the N-terminal element described above to the M HC Class ll-presented epitope described below. The chemical structure is a covalently joined group of atoms which when arranged in a linear fashion for ms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the chemical structure being selected from the group consisting of: i) immunologically neutral chemical structures, ii) a M HC Class I epitope or a portion thereof, and/or iii) an antibody-recognized determinant or a portion thereof. Finally, the enhancing antigen presentatio n enhancing hybrid polypeptide includes a C-terminal element comprising an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecul e.