METHOD FOR PRODUCTION OF VACCINE AGAINST COYPU SALMONELLOSIS

摘要

FIELD: veterinary medicine. ^ SUBSTANCE: claimed method includes sampling of pathological material from fallen coypu from local epizootic focus and preparation of suspension therefrom. Suspension is seeded on differential diagnostic media, and pure culture of salmonella typhimurium is isolated. Pure culture is cultivated in liquid broth with addition of 0.2 % of glucose to produce microbial cell concentration of 4-5x109 per 1 cm3. Culture is inactivated with formalin up to finish concentration of 0.4-0.6 % and conditioned at 37°C for 82-96 hours. Then 3 % solution of aluminum hydroxide is introduced in amount of 15 % based on culture volume, mixture is thoroughly agitated, pre-packed and sealed. ^ EFFECT: specific vaccine of high immunogenic properties. ^ 1 tbl, 1 ex