| 摘要 |
<P>PROBLEM TO BE SOLVED: To enable a single-stranded DNA to remain on a plastic substrate by thermal denaturation after forming a double strand by carrying out a DNA chain elongation reaction on a DNA chip. <P>SOLUTION: A primer for DNA elongation is immobilized to a substrate having a polymer substance, which comprises a first unit having a group induced from a phosphate constituting the hydrophilic moiety of a phospholipid and a second unit having an active ester group, on its surface. Then, a reaction system, into which a template DNA fragment having a desired sequence and a sample containing a nucleotide monomer have been introduced, is heated to a temperature at which the DNA chain is thermally denatured. Next, the temperature of the above reaction system is lowered to a temperature for performing an annealing treatment and the DNA chain elongation is carried out in this reaction system. <P>COPYRIGHT: (C)2006,JPO&NCIPI |
