| 摘要 |
PROBLEM TO BE SOLVED: To provide a simple assay method capable of simply and rapidly specifying a substance to be detected showing positive reaction by testing a plurality of substances to be detected by one assay device in an examination method of a specimen using a flow-through type membrane enzyme immunoassay method and having good user's convenience and high reliability. SOLUTION: A device, which includes a membrane wherein a plurality of capturing reagents which can be bonded to different detection targets, are bonded at mutually separated positions, the filter positioned at the upstream part of the membrane and an enzyme labelling reagent part, which contains a plurality of enzyme labelling reagents capable of being bonded to the different detection targets positioned at the upstream part of the filter, is used. In the assay method, (i) a specimen is added to the enzyme labelling reagent part to form a mixture of the specimen and the enzyme labelling reagent, the mixture is filtered by the filter to be added to the surface of the membrane and the detection target is captured by the capturing reagent to form an immune composite comprising capturing reagent-detection target-enzyme labelling reagent and (ii) a substrate liquid to enzyme is subsequently dripped on the membrane. COPYRIGHT: (C)2006,JPO&NCIPI
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