发明名称 |
Bacterial cell component-unresponsive model mouse |
摘要 |
A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.
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申请公布号 |
US7078585(B1) |
申请公布日期 |
2006.07.18 |
申请号 |
US20010889324 |
申请日期 |
2001.07.13 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
AKIRA SHIZUO;TAKEUCHI OSAMU;TAKEDA KIYOSHI |
分类号 |
A01K67/027;C07K14/705;C12N15/85;C12N15/89;C12Q1/00;C12Q1/02;G01N33/50 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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