Nucleotide analogues comprising a reporter moiety and a polymerase enzyme blocking moiety

摘要

Nucleotides comprising a reporter moiety and a polymerase enzyme blocking moiety in which the reporter moiety does not also act as a polymerase enzyme blocking moiety are described. Also described are compounds of Formula (I): wherein W is a phosphate group, B is a base, Y is a linker comprising an enzyme-cleavable group, R² is a reporter moiety, R³ is selected from H or OH, Z and Z' are selected from H, OH, or a group X-R¹, wherein X is a linker comprising an enzyme-cleavable group and R¹ is a polymerase enzyme blocking group, provided that at least one of Z and Z' is X-R¹.