New polynucleotide encoding insect acyl-CoA-dehydrogenase, useful as a target for identification of insecticides, also new protein, vector and recombinant cells

摘要

Isolated polynucleotide (I) comprising a sequence that encodes a insect acyl-CoA-dehydrogenase polypeptide (II) comprising a defined 418 amino acid sequence (SEQ ID NO:2) given in the specification, is new. Independent claims are also included for: (1) isolated polynucleotide (I) comprising a sequence with at least 75% identity with region 55-1311 of sequence (1), reproduced; (2) isolated polynucleotide (Ib) comprising a sequence that hybridizes under stringent conditions to the 55-1311 region of sequence (1); (3) recombinant vector containing (I), (Ia) or (Ib); (4) recombinant host cell that contains the vector of (3); (5) method for preparing an insect acyl-CoA-dehyrogenase domain (III) by culturing cells of (4); (6) purified protein having a sequence at least 80% identical with sequence (2); and (7) method for detecting an agent (X) that reduces the enzymatic activity of (III). ACTIVITY : Insecticide. No biological data given. MECHANISM OF ACTION : Enzyme Inhibitor. Inhibition of: (a) activity of acyl-CoA dehydrogenase (III); or (b) synthesis of (III) or (III)-encoding mRNA. (III) is essential for alpha ,beta -dehydrogenation of acetyl-CoA esters and for transfer of electrons to the bc1 complex of the respiratory chain, through flavoproteins.