发明名称 |
HIGH YIELD PERTUSSIS VACCINE PRODUCTION STRAIN AND METHOD FOR MAKING SAME |
摘要 |
The present invention provides a vaccine production strain of Bordetella bronchiseptica that produces a pertussis toxin in high yield. The present invention provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin comprising the steps of introducing a plasmid containing a DNA encoding antibiotic resistance into a Bordetella bronchiseptica strain, selecting for isolates in which the DNA encoding antibiotic resistance is recombinantly incorporated into the chromosome in place of the Bordetella bronchiseptica toxin gene, introducing a plasmid containing DNA encoding subunits of the Bordetella pertussis toxins into the Bordetella bronchiseptica isolates; and, selecting for isolates in which DNA encoding Bordetella pertussis toxin subunit is recombinantly incorporated into the chromosome, the resulting cells producing the Bordetella pertussis toxin. The present invention further provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin and does not express filamentous hemagglutinin.
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申请公布号 |
US2006154354(A1) |
申请公布日期 |
2006.07.13 |
申请号 |
US20020312272 |
申请日期 |
2002.12.20 |
申请人 |
MERKEL TOD J;KEITH JERRY M;YANG XIAOMING |
发明人 |
MERKEL TOD J.;KEITH JERRY M.;YANG XIAOMING |
分类号 |
C12N1/21;C07K14/235;C12N15/74 |
主分类号 |
C12N1/21 |
代理机构 |
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主权项 |
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地址 |
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