NEW ULTRA-SENSITIVE CHEMILUMINESCENT SUBSTRATES FOR ENZYMES AND THEIR CONJUGATES

摘要

New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and (1,2)-dioxetanes. Among the new acridane­based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris­acridanes as well as acridane-(1,2)-dioxetanes. Among the new (1,2)-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-l,2-dioxetanes. The (1,2)-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent. An aqueous buffer and , optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.