| 摘要 |
51-Inosinic acid is prepared by a fermentation process which comprises culturing in a culture medium a mixture of (1) a cultured medium obtained from the cultivation in a culture medium of a microorganism which accumulates a substantial amount of hypoxanthine, e.g. a mutant of the species Brevibacterium ammoniagenes, and (2) a cultured medium obtained from the cultivation in a culture medium of a microorganism having a high nucleotide pyrophosphorylase activity of the species Brevibacterium ammoniagenes, and isolating the 51-inosinic acid. The culture media (1) and (2) may be prepared in separate culture media and subsequently combined in a single culture medium for further cultivation, or they may be The culturing is carried out in a medium containing saccharide, nitrogen source and inorganic salt. When necessary, the medium may contain yeast extract, caseate, soybean-cake hydrolyzate, peptone or corn steep liquor. The medium may also contain small amounts of biotin of adenine. The said strains may be inoculated in the seed culture media and cultured at 30 DEG C. for from about 16 to 24 hours. The cultured cells are then transferred to the main culture media in which further culturing with aeration and stirring at from 28 DEG C. for from about 2 to 4 days is carried out, the pH being preferably adjusted to within the range 6.0 to 8.0 with ammonia or urea.
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