New polynucleotide encoding insect diacylglycerol kinase, useful as a target for identification of insecticides, also new protein, vector and recombinant cells

摘要

Isolated polynucleotide (I) comprising a sequence that encodes a polypeptide (II) of sequence (2) of 918 amino acids (aa), reproduced, is new. Independent claims are also included for the following: (1) isolated polynucleotide (I) comprising a sequence with at least 75% identity with region 112-2868 of sequence (1), reproduced; (2) isolated polynucleotide (Ib) comprising a sequence that hybridizes under stringent conditions to the 112-2868 region of sequence (1); (3) recombinant vector containing (I), (Ia) or (Ib); (4) recombinant host cell that contains the vector of (3); (5) preparing an insect diacylglycerol kinase (III) by culturing cells of (4); (6) purified protein having a sequence at least 80% identical with sequence (2); and (7) detecting an agent (X) that reduces the enzymatic activity of (III). ACTIVITY : Insecticide. No biological data given. MECHANISM OF ACTION : Diacylglycerol Kinase Inhibitor (III); Synthesis of (III) or (III)-encoding mRNA Inibitor. (III) is involved in moderating the activity of protein kinase C and thus the intracellular concentration of the second messengers diacylglycerol and phosphatidic acid.