| 摘要 |
PROBLEM TO BE SOLVED: To provide highly heat-resistant DNA polymerase having 3'-5' exonuclease activity being a calibration function of DNA chain elongation and a strong primer elongation activity. SOLUTION: Disclosed is a thermophilic archaebacteria-derived heat-resistant heterodimer enzyme featured by that the enzyme consists of a small subunit and a large subunit having molecular weight of about 70±7 kDa and about 144±14.4 kDa respectively, the large subunit does not contain an intein sequence, at least about 50% of activity is maintained after heating treatment at 85°C for 1 hr, at least about 20% of activity is maintained after heating treatment at 90°C for 1 hr, and the enzyme has DNA polymerase activity and 3'-5' exonuclease activity. Also, a DNA encoding the enzyme and a method for producing the enzyme are disclosed. COPYRIGHT: (C)2006,JPO&NCIPI
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