| 摘要 |
<p>Obtaining a transplastomic plant free of selectable marker comprises transforming at least one plant cell with a vector for the transformation of plastids. Obtaining a transplastomic plant free of selectable marker comprises: (a) transforming at least one plant cell with a vector for the transformation of plastids, comprising, in the direction of transcription, a sequence (i) corresponding to the 5' portion of a chimeric gene of interest, a chimeric gene (ii) comprising a sequence encoding a selectable marker that confers resistance to a selection agent, a fragment (iii) of n nucleotides that is identical to the 3' portion of the sequence (i), and a sequence (iv) corresponding to the remaining 3' portion of the chimeric gene of interest; (b) culturing the cells comprising the transformed plastids on a first medium comprising the selection agent; and (c) culturing the cells on a second medium that does not comprise the selection agent. Independent claims are also included for: (1) a construct comprising, in the direction of transcription, a sequence (i) corresponding to the 5' portion of a chimeric gene of interest, a chimeric gene (ii) comprising a sequence encoding a selectable marker, a fragment (iii) of n nucleotides that is identical to the 3' portion of the sequence (i), and a sequence (iv) corresponding to the remaining 3' portion of the chimeric gene of interest; (2) a transformation vector, for the transformation of plant plastids, comprising the construct; (3) a transplastomic plant cell containing a gene of interest and free of selectable marker, that can be obtained by means of the new method; and (4) a transplastomic plant or a part of this plant, and the progeny of this plant, containing a gene of interest and free of selectable marker, that can be obtained by means of the new method. ACTIVITY : Plant Protectant. MECHANISM OF ACTION : None given.</p> |
