发明名称 |
VERFAHREN UND TRÄGER ZUR BIOLOGISCHEN ANALYSE UNTER VERWENDUNG VON EINEN ENZYMATISCH AKTIVIERBAREN MARKER TRAGENDEN OLIGONUKLEOTIDEN |
摘要 |
The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps: a) contacting the targets to be analyzed (ICAM-20) with oligonucleotide probes (ISIS) labeled with a cofactor A of an enzyme E; b) adding, to the reaction medium, enzyme E corresponding to cofactor A and a substrate S of enzyme E, substrate S being converted by enzyme E into a compound C; c) measuring a signal representative of the activity of enzyme E on substrate S, for example the fluorescence intensity (I) of substrate S; and d) comparing this signal with the signal obtained when the oligonucleotide probes labeled with cofactor A (ISIS) are contacted with enzyme E and substrate S, under the same conditions, but in the absence of the targets, the difference between the two signals indicating the presence of complementary targets (ICAM-20) of the oligonucleotide probes. |
申请公布号 |
DE60211906(D1) |
申请公布日期 |
2006.07.06 |
申请号 |
DE2002611906 |
申请日期 |
2002.07.12 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE, PARIS;UNIVERSITE JOSEPH FOURIER, GRENOBLE |
发明人 |
DECOUT, JEAN-LUC;FONTECAVE, MARC;DUEYMES, CECILE |
分类号 |
C12Q1/68;G01N33/53;C12N15/09;C12Q1/26;G01N21/78;G01N33/566;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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