| 摘要 |
In accordance with the present invention, method are presented for labeling a cDNA strand with a photochemical cleavable reagent which upon exposure to electromagnetic radiation of particular reagent to create abasic DNA sites. According to one aspect of the present invention, DNA at the abasic sites, also known a chemical lactone group, is cleaved with an endonuclease, for example an endonuclease IV, which cleaves the DNA and leaves a free 3' OH group. This free 3' OH group is then labeled with a terminal transferase to provide a detectable moiety. In accordance with a preferred aspect of the present invention,
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