METHODS, COMPOSITIONS, AND KITS FOR FORMING LABELED POLYNUCLEOTIDES

摘要

The present teachings provide novel methods, compositions, and kits for forming multi-labeled polynucleotides. In some embodiments of the present teachings, multiplexed amplification reactions are performed with a plurality of primer pairs, wherein one primer in a given primer pair comprises a distinct label. Additional labeling of the resulting amplicons can be accomplished by using at least one bridge oligonucleotide to ligate a labeled tag oligonucleotide to each labeled extension product, thereby forming a plurality of multi-labeled polynucleotides. Detection of labels such as florophores and mobility modifiers in the plurality of multi-labeled polynucleotides can identify a sample. Such sample identification can be performed using a mobility dependent analysis technique such as capillary electrophoresis, and can applicable in the field of forensics.