发明名称 |
Novel real-time rt-pcr for the sensitive detection of multiple mage gene transcripts |
摘要 |
The present invention relates to a highly sensitive real-time RT-PCR method for specifically detecting the expression of more than one MAGE gene. The present invention further relates to a diagnostic composition for carrying out such a real-time RT-PCR as well as to oligonucleotides suitable for the cDNA synthesis reaction prior to real-time PCR amplification of more than one marker from the MAGE gene family. To enable the quantitative measurement of MAGE gene expression in a clinical sample an RT-protocol was invented using very sophisticated non-standard conditions to accomplish real-time PCR amplification of cDNA of several MAGE family members in relation to a comparative normalizing reference gene as internal control.
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申请公布号 |
US2006147928(A1) |
申请公布日期 |
2006.07.06 |
申请号 |
US20050536932 |
申请日期 |
2005.11.22 |
申请人 |
KUFER PETER;MECKLENBURG INGO |
发明人 |
KUFER PETER;MECKLENBURG INGO |
分类号 |
C12Q1/68;C07K14/47;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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