摘要 |
Disclosed are compounds of formula 1, wherein; R1 is a heteroaromatic group containing 1-5 nitrogen atoms, R2 is H, alkyl or a saccharide moiety, X is oxygen or sulphur, R3 is a heteroaromatic or optionally substituted alkyl, cycloalkyl or heterocyclyl group, R4 is a linker, and L is a label, plurality of labels, a bond connecting R4 to R1 forming a cyclic substrate, or a further group-R3-CH2-X-R1-R2, Also disclosed is a method for detecting and manipulating a protein of interest, wherein the protein of interest incorporated into a AGT fusion protein is contacted with an AGT substrate carrying a label, and the AGT fusion protein is detected and optionally further manipulated using a label in a system designed for recognising or handling the label, and wherein the AGT substrate carrying the label is a compound of formula 1 as above.
|