| 摘要 |
<P>PROBLEM TO BE SOLVED: To establish and provide a method for simply amplifying the objective gene without separating and purifying a nucleic acid from feces as opposed to the conventional methods. <P>SOLUTION: The method for amplifying the gene obtained from the feces comprises (a) a step of dissolving the feces with a dissolving liquid, (b) a step of soaking filter paper with the resultant dissolved liquid and (c) a step of directly bringing the resultant filter paper into contact with a reaction solution for the gene amplification and carrying out the gene amplification. Since the nucleic acid contained in the feces can be separated and adsorbed with the filter paper and the filter paper can directly be used for the gene amplification reaction, the objective gene in the feces can simply and efficiently be amplified without passing through the separation and purification of the nucleic acid as opposed to the conventional methods. <P>COPYRIGHT: (C)2006,JPO&NCIPI |
