发明名称 |
EXPRESSION OF POLYPEPTIDES IN CHLOROPLASTS, AND COMPOSITIONS AND METHODS FOR EXPRESSING SAME |
摘要 |
Method of producing one or more polypeptides in a plant chloroplast, including methods of producing polypeptides that specifically associate in a plant chloroplast to generate a functional protein complex, are provided. An isolated polynucleotide that includes (or encodes) a first ribosome binding sequence (RBS) operatively linked to a second RBS, such that the first RBS directs translation of a polypeptide in a prokaryote and the second RBS directs translation of the polypeptide in a chloroplast, also is provided, as is a vector containing such a polynucleotide, particularly a chloroplast vector and a chloroplast/prokaryote shuttle vector. Also provided is a synthetic polynucleotide, which is chloroplast codon biased. A plant cell that is genetically modified to contain a polynucleotide or vector as described above, as well as transgenic plants containing or derived from such a genetically modified cell, are provided. Polypeptides encoded by a synthetic polynucleotide as described also are provided. |
申请公布号 |
WO03091413(A3) |
申请公布日期 |
2006.06.29 |
申请号 |
WO2003US12997 |
申请日期 |
2003.04.23 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE;MAYFIELD, STEPHEN, P.;FRANKLIN, SCOTT |
发明人 |
MAYFIELD, STEPHEN, P.;FRANKLIN, SCOTT |
分类号 |
A01H5/00;A61K48/00;C07H21/04;C07K14/47;C07K16/00;C07K16/08;C07K19/00;C12N;C12N1/00;C12N1/13;C12N5/04;C12N5/10;C12N15/09;C12N15/62;C12N15/63;C12N15/82;C12P21/00;C12P21/02;C12Q1/02;C12Q1/68 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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