HEAT-RESISTANT THIOREDOXIN, HEAT-RESISTANT THIOREDOXIN REDUCTASE AND DNA ENCODING THESE ENZYMES

摘要

PROBLEM TO BE SOLVED: To provide a heat-resistant thioredoxin and heat-resistant thioredoxin reductase, as well as DNAs encoding these enzymes.

SOLUTION: A thioredoxin and a thioredoxin reductase are isolated from super thermophilic archaebacteria Pyrococcus horikoshii OT3 strain which can grow at a high temperature such as 90 to 100°C, and their amino acid sequences and DNA sequences are determined. These proteins or enzymes are extremely superior in their heat resistances and their activities do not substantially decrease even when they are treated at 100°C for 0.5 hours. The thioredoxin and the like of the invention can effectively perform reaction at a high temperature because their heat resistance is high, and heat sterilization is possible when they are added to pharmaceuticals, foods and cosmetics.

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