METHOD OF DETECTING GENE METHYLATION AND METHOD OF EXAMININGNEOPLASM BY DETECTING METHYLATION

摘要

It is intended to provide a method of conveniently and economically detecting
the methylation of a gene contained in a biological sample. It is also
intended to provide an accurate and sensitive examination method to be used in
the case of examining a neoplasm by using the method of detecting gene
methylation. In examining the methylation of a gene, a polysaccharide is added
to a biological sample before treating it with a bisulfite. Thus, the
subsequent bisulfite treatment can be conducted while requiring neither the
addition of a protein-denaturing agent nor the purification of DNA. Thus
methylated cytosine without uracil-modification can be detected. Moreover, a
neoplasm can be examined at an elevated accuracy by detecting the methylation
of genes of multiple species contained in a biological sample and calculating
the sum thereof.