发明名称 |
Methods of treating microbial infections in humans and animals |
摘要 |
A method of treating a subject with a microbially-based infection, comprising the administration of a compound to the subject. The compound is able to decrease ATP levels in the microbe by at least 10% compared to controls after 24 hours in an in vitro test, without killing mammalian cells during the same time period. The decrease in ATP levels is measured by: (1) removing the cells from the testing location and putting them on ice; (2) harvesting the cells at 4 degrees C. by centrifugation and disrupting it with bead-beating in an ATP extraction buffer; (3) removing cellular debris by centrifugation at 4 degrees C., leaving an ATP-containing supernatant; (4) measuring the amount of ATP present in the supernatant by a bioluminescence assay at 4 degrees C.
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申请公布号 |
US2006135568(A1) |
申请公布日期 |
2006.06.22 |
申请号 |
US20050520506 |
申请日期 |
2005.11.01 |
申请人 |
TOWNSEND CRAIG A;DICK JAMES D;PARRISH NICOLE M;HUGHES MINERVA A |
发明人 |
TOWNSEND CRAIG A.;DICK JAMES D.;PARRISH NICOLE M.;HUGHES MINERVA A. |
分类号 |
A61K31/455;C07D333/32;A61K31/16;A61K31/165;A61K31/22;A61K31/221;A61K31/223;A61K31/341;A61K31/365;A61K31/381;A61K31/39;A61K31/421;A61K31/4409;A61K45/00;A61P31/00;A61P31/04;A61P31/06;A61P31/08;A61P43/00;C07D213/86;C07D263/10;C07D307/68;C07D409/12 |
主分类号 |
A61K31/455 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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