| 摘要 |
<p>1,226,665. Antibiotic T-2636. TAKEDA YAKUHIN YOGYO K.K. 15 May, 1968 [18 May, 1967; 8 May, 1968], No. 23032/68. Heading C2A. [Also in Division A5] The antibiotic T-2636 and its components A, B, C and D, a pentaene having antibacterial activity, is produced by cultivating Streptomyces rochei var. volubilis, especially strain IFO-12507, in a culture medium containing assimilable sources of carbon and nitrogen under aerobic conditions. The antibiotics may be obtained from the clarified broth or mycelium. The whole broth is adjusted to pH 3 to 8, preferably 5 to 6, filtered with a filter aid, and extracted at pH 2 to 9 and preferably 7.0 with a water-immiscible solvent such as ethyl acetate. The extract is treated with aqueous acid of pH 2 to 5, aqueous alkali of pH 8 to 10 and water and the crude antibiotic is precipitated by the addition of petroleum ether or hexane. The separated mycelium may be extracted with 70% aqueous acetone or 70% aqueous methanol, the extract treated with a solvent such as benzene and the crude antibiotic recovered therefrom. Purification is by chromatography on silica gel, elution with benzene or diethyl ether to remove impurities and then elution with chloroform/ methanol (95: 5). Separation into components is obtained by chromatography on silica gel followed by development using various solvent systems, e.g. benzene/ethyl acetate (8: 2), (7:3), (6:4), (5:5), (4:6), (3:7), (2:8) and ethyl acetate respectively. The antibiotic T-2636 components or mixtures thereof may be incorporated in ointments for the treatment of staphylococcal infections of humans and animals or may be dissolved in methanol or ethanol as a general disinfectant.</p> |
