Cloning and characterisation of the gene flbA of H. pylori; production of flagella-free strains

摘要

A nucleotide sequence regulating flagellate protein biosynthesis in Helicobacter pylori (H. pylori) is capable of hybridising in conditions of great stringency with a specillum corresponding to a fragment of H. pylori nucleotides amplified with the aid of two oligonucleotides corresponding to specific sequences. One such sequence can be obtained by screening a genome bank containing the chromosome DNA of a branch of H. pylori with the above specillum, recuperating the DNA sequences that hybridise with the specillum, sub-cloning the DNA sequences obtained in an appropriate plasmide-type vector and selecting modified vectors that hybridise in conditions of great stringency with the specillum.