METHOD AND SYSTEM FOR PEAK PARKING IN LIQUID CHROMATOGRAPHY-MASS SPECTROMETER (LC-MS) ANALYSIS

摘要

A reconfigurable multi-mode post-column analysis system improves resolution of liquid chromatography detection data by providing a parking or MS/MS detecti on mode in which flow of column eluent is substantially reduced to provide greater analysis time within a single eluting detection peak. A micro switching valve unit is reconfigurable between normal (MS) mode that passes column eluent directly to an MS unit, and a peak parking (or MS/MS) mode, in which normal mode column flow is interrupted, and the gradient halted, while more slowly flowing the detection peak of interested through the MS unit. A micro syringe pump contributes to the substantially lower MS/MS flow rate. A MS control unit controls micro switching valve valves to reconfigure between MS and MS/MS modes. After a detection peak, MS mode is reestablished, and th e system primary pump returns to normal mode flow rate and gradient conditions . Mode changes can be carried out for each chromatogram detection peak.