发明名称 |
СПОСОБ ВЫЯВЛЕНИЯ ПЕРЕКРЕСТНО РЕАГИРУЮЩИХ АНТИГЕНОВ ВОЗБУДИТЕЛЕЙ МЕЛИОИДОЗА, САПА, ЧУМЫ, ТУЛЯРЕМИИ ИТУБЕРКУЛЕЗА |
摘要 |
FIELD: medicine, laboratory diagnostics. ^ SUBSTANCE: the present innovation deals with elaborating efficient method for detecting common and cross-reacting antigens in Burkholderia pseudomallei, Burkholderia mallei, Yersinia pestis, Francisella tularensis and mycobacterium tuberculosis at simultaneous detection of their main physico-chemical characteristics. It is necessary to carry out adsorption of cross-reacting antigens upon immunocomplexone IgGxAl(OH)3 at additional IgG precipitation with staphylococcal reagent with protein A followed by detection of molecular weights of cross-reacting antigens due to analyzing the obtained adsorbed and nonadsorbed preparations in SDS-PAAG electrophoresis and immunoblotting. The innovation enables to elaborate new high-sensitive method for detecting the cluster of cross-reacting antigens in the agents of melioidosis, glanders, plague, tularemia and tuberculosis along with establishing their main physico-chemical characteristics. ^ EFFECT: higher efficiency and accuracy of detection. ^ 2 ex |
申请公布号 |
RU2004137405(A) |
申请公布日期 |
2006.06.10 |
申请号 |
RU20040137405 |
申请日期 |
2004.12.21 |
申请人 |
Волгоградский научно-исследовательский противочумный институт (RU) |
发明人 |
Пивень Николай Николаевич (RU);Тимошин Валерий Борисович (RU);Алексеев Владимир Валерьевич (RU);Илюхин Владимир Иванович (RU);Викторов Дмитрий Викторович (RU);Плеханова Наталь Геннадьевна (RU);Вобленко Роман Александрович (RU) |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
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